Device

Part:BBa_K3702192:Design

Designed by: Dat Vo, Christine Cremeans   Group: iGEM20_FSU   (2020-09-28)


ParE2 generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 340
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This device was designed to be part of a kill switch along with another generator producing the ParD2 antitoxin. The purpose of the kill switch was to kill the host cell (E. coli) when the host cell get out of the wastewater treatment plant.

Source

https://parts.igem.org/Part:BBa_J23118 https://parts.igem.org/Part:BBa_B0034 https://parts.igem.org/Part:BBa_K2150104 https://parts.igem.org/Part:BBa_B0015

References